ARG82721
Mouse Serpin D1 / Heparin cofactor II ELISA Kit
Mouse Serpin D1 / Heparin cofactor II ELISA 试剂盒 for ELISA and Mouse
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82721-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82721-002 | Standard | 2 X 50 ng/vial | 4°C |
| ARG82721-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82721-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82721-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82721-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82721-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82721-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82721-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82721-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82721-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82721 Mouse Serpin D1 / Heparin cofactor II ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Serpin D1 / Heparin cofactor II in serum, plasma (EDTA, citrate), cell culture supernatants and urine. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 靶点名称 | Serpin D1 / Heparin cofactor II |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 0.39 ng/ml |
| 样品类型 | Serum, plasma (EDTA, citrate), cell culture supernatants and urine. |
| 标准范围 | 0.78 - 50 ng/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.9% Inter-Assay CV: 6.3% |
| 別名 | HC2; Protease inhibitor leuserpin-2; D22S673; HC-II; HCII; THPH10; Heparin cofactor 2; Heparin cofactor II; HCF2; LS2; HLS2; Serpin D1 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SERPIND1 |
| 全名 | serpin peptidase inhibitor, clade D (heparin cofactor), member 1 |
| 背景介绍 | This gene belongs to the serpin gene superfamily. Serpins play roles in many processes including inflammation, blood clotting, and cancer metastasis. Members of this family have highly conserved secondary structures with a reactive center loop that interacts with the protease active site to inhibit protease activity. This gene encodes a plasma serine protease that functions as a thrombin and chymotrypsin inhibitor. The protein is activated by heparin, dermatan sulfate, and glycosaminoglycans. Allelic variations in this gene are associated with heparin cofactor II deficiency. [provided by RefSeq, Jul 2015] |
| 生物功能 | Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner. Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils. [UniProt] |
| 产品亮点 | Related products: Serpin antibodies; Serpin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Phosphorylated by FAM20C in the extracellular medium. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG82721 Mouse Serpin D1 / Heparin cofactor II ELISA Kit standard curve image
ARG82721 Mouse Serpin D1 / Heparin cofactor II ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82721 Mouse Serpin D1 / Heparin cofactor II ELISA Kit User manual |
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