ARG81672
Mouse SCF ELISA Kit
Mouse SCF ELISA 试剂盒 for ELISA and Mouse
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81672-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81672-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81672-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81672-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81672-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81672-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81672-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81672-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81672-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81672-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81672-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81672 Mouse SCF ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse SCF in serum, plasma (EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | SCF |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (EDTA) and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.9%; Inter-Assay CV: 6.9% |
| 別名 | SCF; SHEP7; MGF; FPHH; sKITLG; Stem cell factor; KL-1; Kitl; Kit ligand; Mast cell growth factor; c-Kit ligand; FPH2; SF |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | KITLG |
|---|---|
| 全名 | KIT ligand |
| 背景介绍 | This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins. [UniProt] |
| 产品亮点 | Related products: SCF antibodies; SCF ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain. Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97. A soluble form exists as a cleavage product of the extracellular domain. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG81672 Mouse SCF ELISA Kit standard curve image
ARG81672 Mouse SCF ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81672 Mouse SCF ELISA Kit User's manual |
 Download
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