ARG82529
Mouse PODXL ELISA Kit
Mouse PODXL ELISA 试剂盒 for ELISA and Mouse
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82529-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82529-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82529-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82529-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82529-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82529-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82529-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82529-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82529-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82529-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82529-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82529 Mouse PODXL ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse PODXL in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 靶点名称 | PODXL |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.1% Inter-Assay CV: 6.9% |
| 別名 | Gp200; GCTM-2 antigen; PCLP; Podocalyxin; PC; PCLP-1; Podocalyxin-like protein 1 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PODXL |
| 全名 | podocalyxin-like |
| 背景介绍 | This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008] |
| 生物功能 | Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up inital epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells. [UniProt] |
| 细胞定位 | Apical cell membrane. Cell projection, lamellipodium. Cell projection, filopodium. Cell projection, ruffle. Cell projection, microvillus. Membrane raft. Membrane; Single-pass type I membrane protein. [UniProt] |
| 产品亮点 | Related products: PODXL antibodies; PODXL ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | N- and O-linked glycosylated. Sialoglycoprotein (By similarity). [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG82529 Mouse PODXL ELISA Kit standard curve image
ARG82529 Mouse PODXL ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82529 Mouse PODXL ELISA Kit User manual |
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