ARG65326
Mouse IgG3 Isotype Control antibody [PPV-07]
Mouse IgG3 Isotype Control 抗体 [PPV-07] for Control,Flow cytometry
Controls and Markers antibody
概述
| 产品描述 | Mouse Monoclonal antibody [PPV-07] as a negative control antibody for Mouse IgG3 |
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| 不反应物种 | Hu |
| 应用 | Control, FACS |
| 特异性 | This mouse IgG3 monoclonal antibody (clone PPV-07) reacts with undefined epitope on a plant pathogen. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | PPV-07 |
| 同位型 | IgG3 |
| 靶点名称 | Mouse IgG3 |
| 偶联标记 | Un-conjugated |
应用说明
| 交叉反应性说明 | Negative Species: Human, Pig, Mouse, Rat, Other species Not tested (expected negative) | ||||||
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| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from hybridoma culture supernatant by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 背景介绍 | The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μg/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody PPV-07, generated against a plant pathogen, does not cross-react with other species, and hence all the background that could be observed when working with this antibody would be a result of general nonspecific interactions between an mouse IgG3 molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished. |
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| 研究领域 | Controls and Markers antibody |
