ARG42262
Mouse IgG2a Isotype Control antibody [MOPC-173] (low endotoxin)
Mouse IgG2a Isotype Control 抗体 [MOPC-173] (low endotoxin) for Control,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot
概述
| 产品描述 | Azide free and low endotoxin Mouse Monoclonal antibody [MOPC-173] as a negative control antibody for Mouse IgG2a |
|---|---|
| 不反应物种 | Hu, Ms, Rat |
| 应用 | Control, FACS, ICC/IF, IHC-P, IP, WB |
| 特异性 | This mouse IgG2a monoclonal antibody (clone MOPC-173) reacts with an unknown epitope. It does not react with a variety of resting, activated, live, and fixed mouse, rat and human tissues. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | MOPC-173 |
| 同位型 | IgG2a, kappa |
| 靶点名称 | Mouse IgG2a |
| 抗原 | Induced by intraperitoneal injection of mineral oils into BALB/c mice. |
| 偶联标记 | Un-conjugated |
应用说明
| 应用建议 |
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| 应用说明 | Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein A. |
| 纯化说明 | 0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein. |
| 缓冲液 | PBS |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
检测图片 (1) Click the Picture to Zoom In
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ARG42262 Mouse IgG2a Isotype Control antibody [MOPC-173] (low endotoxin) FACS image
Flow Cytometry: ARG42262 Mouse IgG2a Isotype Control antibody [MOPC-173] (low endotoxin), followed by APC-conjugated Goat anti-Mouse antibody (red) on Human peripheral blood compared with blank (black).
