ARG70180
Mouse IL33 recombinant protein (Active) (His-tagged, C-ter)
Mouse IL33 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
概述
| 产品描述 | E. coli expressed, His-tagged (C-ter) Active Mouse IL33 recombinant protein |
|---|---|
| 应用 | SDS-PAGE |
| 靶点名称 | IL33 |
| 物种 | Mouse |
| A.A. 序列 | Ser109 - Ile266 |
| 表达系统 | E. coli |
| 生物活性 | Active |
| 活性说明 | Determined by its ability to induce proliferation in D10.G4.1 cells. The ED50 for this effect is < 40 pg/mL. The specific activity of recombinant mouse IL-33 is > 2 x 10^7 IU/mg. |
| 別名 | 95-270; NF-HEV; Interleukin-33; C9orf26; IL1F11; 99-270; Interleukin-1 family member 11; IL-33; IL-1F11; Nuclear factor from high endothelial venules; NFEHEV; DVS27; 109-270 |
属性
| 形式 | Powder |
|---|---|
| 纯化说明 | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 98% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 复溶 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | IL33 |
|---|---|
| 全名 | interleukin 33 |
| 背景介绍 | The protein encoded by this gene is a cytokine that binds to the IL1RL1/ST2 receptor. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] |
| 生物功能 | Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury. In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation. [UniProt] |
| 细胞定位 | Nucleus. Chromosome. Cytoplasmic vesicle, secretory vesicle. Secreted. Note=Associates with heterochromatin and mitotic chromosomes (PubMed:17185418). [UniProt] |
| 翻译后修饰 | The full length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains (PubMed:19596270). Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:19559631). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase. [UniProt] |
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ARG70180 Mouse IL33 recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70180 Mouse IL33 recombinant protein (Active) (His-tagged, C-ter).
