ARG70151

Mouse IL1 alpha recombinant protein (Active) (His-tagged, C-ter)

Mouse IL1 alpha recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE

概述

产品描述 E. coli expressed, His-tagged (C-ter) Active Mouse IL1 alpha recombinant protein
应用 SDS-PAGE
靶点名称 IL1 alpha
物种 Mouse
A.A. 序列 MSAPY TYQSD LRYKL MKLVR QKFVM NDSLN QTIYQ DVDKH YLSTT WLNDL QQEVK FDMYA YSSGG DDSKY PVTLK ISDSQ LFVSA QGEDQ PVLLK ELPET PKLIT GSETD LIFFW KSINS KNYFT SAAYP ELFIA TKEQS RVHLA RGLPS MTDFQ IS with polyhistidine tag at the C-terminus.
表达系统 E. coli
活动 Active
活动说明 Determined by its ability to induce D10.G4.1 cells proliferation. The ED50 for this effect is < 5 pg/ml. The specific activity of recombinant mouse IL-1 alpha is > 2 x 10^8 IU/mg.
別名 IL-1 alpha; Interleukin-1 alpha; IL1; IL1F1; Hematopoietin-1; IL1-ALPHA; IL-1A

属性

形式 Powder
纯化 Ni-NTA chromatography.
纯化说明 Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
纯度 > 95% (by SDS-PAGE)
缓冲液 PBS (pH 7.4)
重建 It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
存放说明 Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

基因名称 IL1A
全名 interleukin 1, alpha
背景介绍 The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]
生物功能 Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. [UniProt]
细胞定位 Secreted. Note=The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. [UniProt]
产品亮点 Related products:
IL1 alpha antibodies; IL1 alpha ELISA Kits; IL1 alpha recombinant proteins;
Related news:
HMGB1 in inflammation
Inflammatory Cytokines

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  • ARG70151 Mouse IL1 alpha recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image

    SDS-PAGE analysis of ARG70151 Mouse IL1 alpha recombinant protein (Active) (His-tagged, C-ter).