ARG70189
Mouse FGF basic recombinant protein (Active) (His-tagged, N-ter)
Mouse FGF basic recombinant protein (Active) (His-tagged, N-ter) for SDS-PAGE
概述
| 产品描述 | E. coli expressed, His-tagged (N-ter) Active Mouse FGF basic recombinant protein |
|---|---|
| 应用 | SDS-PAGE |
| 靶点名称 | FGF basic |
| 物种 | Mouse |
| A.A. 序列 | Ala11 - Ser154 |
| 表达系统 | E. coli |
| 生物活性 | Active |
| 活性说明 | Determined by its ability to induce 3T3 cells proliferation. The ED50 for this effect is < 1.5 ng/mL. The specific activity of recombinant mouse FGF-2 is approximately >1x 10^6 IU/mg. |
| 別名 | FGF-2; Fibroblast growth factor 2; bFGF; FGFB; Heparin-binding growth factor 2; BFGF; HBGF-2; Basic fibroblast growth factor |
属性
| 形式 | Powder |
|---|---|
| 纯化说明 | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 98% (by SDS-PAGE) |
| 缓冲液 | PBS containing 0.01% sarkosyl (pH 8.0) |
| 复溶 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | FGF2 |
|---|---|
| 全名 | fibroblast growth factor 2 (basic) |
| 背景介绍 | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008] |
| 生物功能 | Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. [UniProt] |
| 细胞定位 | Secreted. Nucleus. Note=Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57. [UniProt] |
| 产品亮点 | Related products: FGF basic antibodies; FGF basic ELISA Kits; FGF basic recombinant proteins; Related news: The role of HDGF in tumor angiogenesis |
| 翻译后修饰 | Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion. Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. [UniProt] |
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ARG70189 Mouse FGF basic recombinant protein (Active) (His-tagged, N-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70189 Mouse FGF basic recombinant protein (Active) (His-tagged, N-ter).
