ARG82549
Mouse Cadherin 12 ELISA Kit
Mouse Cadherin 12 ELISA 试剂盒 for ELISA and Mouse
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82549-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82549-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82549-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82549-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82549-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82549-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82549-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82549-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82549-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82549-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82549-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82549 Mouse Cadherin 12 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse Cadherin 12 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 靶点名称 | Cadherin 12 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 4.2% Inter-Assay CV: 7.1% |
| 別名 | Cadherin-12; Brain cadherin; CDHB; Neural type cadherin 2; BR-cadherin; N-cadherin 2 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CDH12 |
| 全名 | cadherin 12, type 2 (N-cadherin 2) |
| 背景介绍 | This gene encodes a type II classical cadherin from the cadherin superfamily of integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. This particular cadherin appears to be expressed specifically in the brain and its temporal pattern of expression would be consistent with a role during a critical period of neuronal development, perhaps specifically during synaptogenesis. [provided by RefSeq, Jul 2008] |
| 生物功能 | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. [UniProt] |
| 产品亮点 | Related products: Cadherin antibodies; Cadherin ELISA Kits; Cadherin Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1) Click the Picture to Zoom In
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ARG82549 Mouse Cadherin 12 ELISA Kit standard curve image
ARG82549 Mouse Cadherin 12 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82549 Mouse Cadherin 12 ELISA Kit User manual |
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