ARG70199
Mouse CNTF recombinant protein (Active) (His-tagged, C-ter)
Mouse CNTF recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
概述
| 产品描述 | E. coli expressed, His-tagged (C-ter) Active Mouse CNTF recombinant protein |
|---|---|
| 应用 | SDS-PAGE |
| 靶点名称 | CNTF |
| 物种 | Mouse |
| A.A. 序列 | Met1 - Met198 |
| 表达系统 | E. coli |
| 生物活性 | Active |
| 活性说明 | Determined by its ability to induce proliferation in TF-1 cells. The ED50 for this effect is < 10 ng/mL. The specific activity of recombinant mouse CNTF is > 1 x 10^5 IU/mg. |
| 別名 | HCNTF; CNTF; Ciliary neurotrophic factor |
属性
| 形式 | Powder |
|---|---|
| 纯化说明 | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 98% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 复溶 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | CNTF |
|---|---|
| 全名 | ciliary neurotrophic factor |
| 背景介绍 | The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of the upstream ZFP91 gene and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. [provided by RefSeq, Oct 2010] |
| 生物功能 | CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG70199 Mouse CNTF recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70199 Mouse CNTF recombinant protein (Active) (His-tagged, C-ter).
