ARG82487
Mouse CHIT1 ELISA Kit
Mouse CHIT1 ELISA 试剂盒 for ELISA and Mouse
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82487-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82487-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82487-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82487-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82487-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82487-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82487-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82487-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82487-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82487-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82487-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82487 Mouse CHIT1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CHIT1 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 靶点名称 | CHIT1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 156 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 312 - 20000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.3% Inter-Assay CV: 7.1% |
| 別名 | EC 3.2.1.14; Chitotriosidase-1; CHI3; CHIT; Chitinase-1; CHITD |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CHIT1 |
| 全名 | chitinase 1 (chitotriosidase) |
| 背景介绍 | Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Jan 2012] |
| 生物功能 | Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity. [UniProt] |
| 细胞定位 | Secreted. Lysosome. Note=A small proportion is lysosomal. [UniProt] |
| 产品亮点 | Related products: CHIT1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1) Click the Picture to Zoom In
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ARG82487 Mouse CHIT1 ELISA Kit standard curve image
ARG82487 Mouse CHIT1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82487 Mouse CHIT1 ELISA Kit User manual |
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