ARG81714
Mouse CD27 ELISA Kit
Mouse CD27 ELISA 试剂盒 for ELISA and Mouse
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81714-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81714-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81714-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81714-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81714-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81714-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81714-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81714-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81714-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81714-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81714-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81714 Mouse CD27 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse CD27 in serum and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | CD27 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 31.25 pg/ml |
| 样品类型 | Serum and cell culture supernatants. |
| 标准范围 | 62.5 - 4000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.2%; Inter-Assay CV: 7.1% |
| 別名 | T-cell activation antigen CD27; Tp55; CD antigen CD27; CD27 antigen; S152. LPFS2; T14; S152; Tumor necrosis factor receptor superfamily member 7; CD27L receptor; TNFRSF7 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | CD27 |
| 全名 | CD27 molecule |
| 背景介绍 | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008] |
| 生物功能 | Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1. [UniProt] |
| 产品亮点 | Related products: CD27 antibodies; CD27 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Phosphorylated. O-glycosylated with core 1 or possibly core 8 glycans. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG81714 Mouse CD27 ELISA Kit standard curve image
ARG81714 Mouse CD27 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81714 Mouse CD27 ELISA Kit User's manual |
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