ARG83741
Mouse ADAMTS1 ELISA Kit (Rapid One-Step)
Mouse ADAMTS1 ELISA 试剂盒 (Rapid One-Step) for ELISA and Mouse
概述
| 产品描述 | ARG83741 Mouse ADAMTS1 ELISA Kit (Rapid One-Step) is an Enzyme Immunoassay kit for the quantification of Mouse ADAMTS1 in serum and plasma (heparin), cell culture supernatants. It is a rapid One-step 90 minutes protocol. |
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| 反应物种 | Ms |
| 应用 | ELISA |
| 靶点名称 | ADAMTS1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 10 pg/ml |
| 样品类型 | serum and plasma (heparin), cell culture supernatants |
| 标准范围 | 93.8 pg/ml - 6000 pg/ml |
| 样本量 | 50 μl |
| 精确 | Intra-Assay CV: < 10% Inter-Assay CV: < 10% |
| 別名 | ADAMTS-1; A disintegrin and metalloproteinase with thrombospondin motifs 1; METH-1; METH1; EC 3.4.24.-; ADAM-TS1; C3-C5; ADAM-TS 1 |
应用说明
| 检测时间 | ~ 1.5 hours |
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属性
| 形式 | 96 well |
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| 存放说明 | Store the kit at 4°C, -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P97857 Mouse A disintegrin and metalloproteinase with thrombospondin motifs 1 |
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| 基因名称 | ADAMTS1 |
| 全名 | ADAM metallopeptidase with thrombospondin type 1 motif, 1 |
| 背景介绍 | This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene contains two disintegrin loops and three C-terminal TS motifs and has anti-angiogenic activity. The expression of this gene may be associated with various inflammatory processes as well as development of cancer cachexia. This gene is likely to be necessary for normal growth, fertility, and organ morphology and function. [provided by RefSeq, Jul 2008] |
| 生物功能 | Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture. [UniProt] |
| 细胞定位 | Extracellular matrix; Secreted. [Uniprot] |
| 翻译后修饰 | The precursor is cleaved by a furin endopeptidase. Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion (By similarity). [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG83741 Mouse ADAMTS1 ELISA Kit (Rapid One-Step) standard curve image
ARG83741 Mouse ADAMTS1 ELISA Kit (Rapid One-Step) results of a typical standard for Mouse ADAMTS1 run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG83741 Mouse ADAMTS1 ELISA Kit-User manual |
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