ARG81679
Mouse ACE ELISA Kit
Mouse ACE ELISA 试剂盒 for ELISA and Mouse
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81679-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81679-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81679-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81679-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81679-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81679-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81679-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81679-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81679-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81679-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81679-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81679 Mouse ACE ELISA Kit is an Enzyme Immunoassay kit for the quantification of Mouse ACE in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Ms |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | ACE |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 46.9 pg/ml |
| 样品类型 | Serum, plasma (heparin) and cell culture supernatants. |
| 标准范围 | 93.8 - 6000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.9%; Inter-Assay CV: 6.6% |
| 別名 | DCP1; ICH; ACE; EC 3.2.1.-; MVCD3; Angiotensin-converting enzyme; Dipeptidyl carboxypeptidase I; CD143; CD antigen CD143; EC 3.4.15.1; Kininase II; ACE1; DCP |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ACE |
| 全名 | angiotensin I converting enzyme |
| 背景介绍 | This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, and two most abundant spliced variants encode the somatic form and the testicular form, respectively, that are equally active. [provided by RefSeq, May 2010] |
| 生物功能 | Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. [UniProt] |
| 产品亮点 | Related products: ACE antibodies; ACE ELISA Kits; ACE Duos / Panels; ACE recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Phosphorylated by CK2 on Ser-1299; which allows membrane retention. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG81679 Mouse ACE ELISA Kit standard curve image
ARG81679 Mouse ACE ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81679 Mouse ACE ELISA Kit User's manual |
 Download
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