ARG80804

Lupin ELISA Kit

Lupin ELISA 试剂盒 for ELISA

Immune System kit
publication_link 参考文献1

概述

产品描述 ARG80804 Lupin ELISA Kit is a Enzyme Immunoassay kit for the quantification of Lupin in food (extraction, dilution).
应用 ELISA
靶点名称 Lupin
偶联标记 HRP
偶联标记说明 Substrate: TMB and read at 450 nm
灵敏度 0.2 ppm
样品类型 Food (extraction, dilution).
标准范围 2 - 30 ppm
样本量 100 µl

应用说明

检测时间 20, 20 min (RT), 20 min (RT/dark)

属性

形式 96 well
存放说明 Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

全名 Lupin
背景介绍 Lupin belongs to the legume family. With about 35-45% the fraction of proteins in lupin seed is very high. Some of these proteins, predominantly those in the alpha, beta and gamma-Conglutin fractions are known for being allergenic, due to cross-reaction to allergens of soy and peanut. Because of its convenient nutritional properties the use of lupin flour as a food ingredient is highly increasing. Lupin is often used in dietary products, especially for milk allergic people, vegetarians and patients with celiac disease. In food production three (sweet) lupin species are commonly used: Lupinus albus, Lupinus angustifolius and Lupinus luteus.

For lupin allergic persons hidden lupin allergens in food are a critical problem. Already very low amounts of lupin can cause allergic reactions, which may lead to anaphylactic shock in severe cases. Because of this, lupin allergic per­sons must strictly avoid the consumption of lupin containing food. Cross-contamination, most­ly in consequence of the production pro­cess, is often noticed. This explains why in many cases the existence of lupin residues in food cannot be excluded. For this reason sensitive detection systems for lupin residues in food­stuffs are required.

The Lupin ELISA represents a highly sensitive detection system for lupin and is particularly capable of the quantification of residues in sausage, bakery products, potato products, ketchup and juices. Due to high cross-reactivity the test is suitable for the detection of all commonly used lupin species.
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研究领域 Immune System kit

检测图片 (1) Click the Picture to Zoom In

  • ARG80804 Lupin ELISA Kit standard curve image

    ARG80804 Lupin ELISA Kit results of a typical standard run with optical density reading at 450nm.

文献引用

Lupine allergens: Clinical relevance, molecular characterization, cross-reactivity, and detection strategies

(Review) / (Review)

Caterina Villa et al.
Compr Rev Food Sci Food Saf .,  (2020)

publication_link

 

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Title Download Link
ARG80804 Lupin ELISA Kit User's manual download icon Download