ARG83747
Human alpha Synuclein ELISA Kit
Human alpha Synuclein ELISA 试剂盒 for ELISA and Human
概述
| 产品描述 | ARG83747 Human alpha Synuclein ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human alpha Synuclein in serum, plasma and cell culture supernatants. |
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| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | alpha Synuclein |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 0.31 ng/ml |
| 样品类型 | Serum, plasma and cell culture supernatants. |
| 标准范围 | 0.63 ng/ml - 40 ng/ml |
| 样本量 | 100 μl |
| 精确 | Intra-Assay CV: < 10% Inter-Assay CV: < 10% |
| 別名 | Non-A4 component of amyloid precursor; Alpha-synuclein; PARK4; PARK1; PD1; NACP; Non-A beta component of AD amyloid |
应用说明
| 检测时间 | 3.5 hours |
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属性
| 形式 | 96 well |
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| 存放说明 | Store the kit at 4°C, -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SNCA |
| 全名 | synuclein, alpha (non A4 component of amyloid precursor) |
| 背景介绍 | Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016] |
| 生物功能 | May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. [UniProt] |
| 细胞定位 | Amyloid; Cell projection; Cytoplasm; Membrane; Nucleus; Secreted; Synapse. [UniProt] |
| 翻译后修饰 | Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress. Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers. Ubiquitinated. The predominant conjugate is the diubiquitinated form (By similarity). Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. |
检测图片 (1) Click the Picture to Zoom In
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ARG83747 Human alpha Synuclein ELISA Kit standard curve image
ARG83747 Human alpha Synuclein ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG83747 Human alpha Synuclein ELISA Kit User_manual |
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