ARG82830
Human alpha 1 Antitrypsin ELISA Kit
Human alpha 1 Antitrypsin ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82830-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82830-002 | Standard | 2 X 100 ng/vial | 4°C |
| ARG82830-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82830-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82830-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82830-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82830-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82830-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82830-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82830-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82830-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82830 Human alpha 1 Antitrypsin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human alpha 1 Antitrypsin in serum, plasma (EDTA, heparin), cell culture supernatants and urine. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | alpha 1 Antitrypsin |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 0.78 ng/ml |
| 样品类型 | Serum, plasma (EDTA, heparin), cell culture supernatants and urine. |
| 标准范围 | 1.56 - 100 ng/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 7.0% Inter-Assay CV: 7.9% |
| 別名 | Serpin A1; alpha1AT; Alpha-1-antiproteinase; Alpha-1 protease inhibitor; AAT; A1A; A1AT; PRO2275; Alpha-1-antitrypsin; PI1; PI; SPAAT |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SERPINA1 |
| 全名 | serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 |
| 背景介绍 | The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. [Short peptide from AAT]: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). [UniProt] |
| 细胞定位 | Secreted. Endoplasmic reticulum. Note=The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum. Short peptide from AAT: Secreted, extracellular space, extracellular matrix. [UniProt] |
| 翻译后修饰 | N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant. Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG82830 Human alpha 1 Antitrypsin ELISA Kit standard curve image
ARG82830 Human alpha 1 Antitrypsin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82830 Human alpha 1 Antitrypsin ELISA Kit User manual |
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