ARG81760
Human TrkB ELISA Kit
Human TrkB ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81760-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81760-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81760-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81760-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81760-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81760-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81760-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81760-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81760-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81760-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81760-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81760 Human TrkB ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human TrkB in cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | TrkB |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.7%; Inter-Assay CV: 7.6% |
| 別名 | TRKB; Neurotrophic tyrosine kinase receptor type 2; Trk-B; trk-B; Tropomyosin-related kinase B; TrkB tyrosine kinase; BDNF/NT-3 growth factors receptor; GP145-TrkB; EC 2.7.10.1 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NTRK2 |
| 全名 | neurotrophic tyrosine kinase, receptor, type 2 |
| 背景介绍 | This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] |
| 生物功能 | Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells and mediate communication between neurons and glia. [UniProt] |
| 产品亮点 | Related products: Trk antibodies; Trk ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Phosphorylated. Undergoes ligand-mediated autophosphorylation that is required for interaction with SHC1 and PLCG1 and other downstream effectors. Isoform TrkB-T-Shc is not phosphorylated. Ubiquitinated. Undergoes polyubiquitination upon activation; regulated by NGFR. Ubiquitination regulates the internalization of the receptor (By similarity). [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG81760 Human TrkB ELISA Kit standard curve image
ARG81760 Human TrkB ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81760 Human TrkB ELISA Kit User manual |
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