ARG70241
Human TIGIT recombinant protein (His-tagged, C-ter)
Human TIGIT recombinant protein (His-tagged, C-ter) for Binding Activity,SDS-PAGE and Human
概述
| 产品描述 | HEK293 expressed, His-tagged (C-ter) Human TIGIT recombinant protein. |
|---|---|
| 反应物种 | Hu |
| 应用 | Binding, SDS-PAGE |
| 靶点名称 | TIGIT |
| 物种 | Human |
| A.A. 序列 | Met22 - Pro141 of Human TIGIT (NP_776160.2) with 6X His tag at the C - terminus. |
| 表达系统 | HEK293 |
| 別名 | WUCAM; V-set and transmembrane domain-containing protein 3; V-set and immunoglobulin domain-containing protein 9; VSIG9; VSTM3; T-cell immunoreceptor with Ig and ITIM domains |
应用说明
| 应用说明 | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Human TIGIT at 1.5 ug/ml (100 µl/well) can bind Human CD155 with a linear range of 0.15-1.2ug/ml. |
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属性
| 形式 | Powder |
|---|---|
| 纯化说明 | 0.22 µm filter sterilized. Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 97% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 复溶 | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | TIGIT |
|---|---|
| 全名 | T cell immunoreceptor with Ig and ITIM domains |
| 背景介绍 | This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses. [provided by RefSeq, Sep 2009] |
| 生物功能 | Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. [UniProt] |
| 预测分子量 | 26 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG70241 Human TIGIT recombinant protein (ECD) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70241 Human TIGIT recombinant protein (ECD) (His-tagged, C-ter).
