ARG82533
Human SPINK5 ELISA Kit
Human SPINK5 ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82533-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82533-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82533-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82533-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82533-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82533-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82533-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82533-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82533-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82533-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82533-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82533 Human SPINK5 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human SPINK5 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | SPINK5 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 156 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| 标准范围 | 312 - 20000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.0% Inter-Assay CV: 5.8% |
| 別名 | LETKI; LEKTI; Lympho-epithelial Kazal-type-related inhibitor; Serine protease inhibitor Kazal-type 5; VAKTI; NS; NETS |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q9NQ38 Human Serine protease inhibitor Kazal-type 5 |
|---|---|
| 基因名称 | SPINK5 |
| 全名 | serine peptidase inhibitor, Kazal type 5 |
| 背景介绍 | This gene encodes a multidomain serine protease inhibitor that contains 15 potential inhibitory domains. The inhibitor may play a role in skin and hair morphogenesis and anti-inflammatory and/or antimicrobial protection of mucous epithelia. Mutations may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 生物功能 | Serine protease inhibitor, probably important for the anti-inflammatory and/or antimicrobial protection of mucous epithelia. Contribute to the integrity and protective barrier function of the skin by regulating the activity of defense-activating and desquamation-involved proteases. Inhibits KLK5, it's major target, in a pH-dependent manner. Inhibits KLK7, KLK14 CASP14, and trypsin. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 产品亮点 | Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Proteolytically processed by furin in individual domains (D1, D5, D6, D8 through D11, and D9 through D15) exhibiting various inhibitory potentials for multiple proteases. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG82533 Human SPINK5 ELISA Kit standard curve image
ARG82533 Human SPINK5 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82533 Human SPINK5 ELISA Kit User manual |
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