ARG81938
Human SPHK1 ELISA Kit
Human SPHK1 ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81938-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81938-002 | Standard | 2 X 20 ng/vial | 4°C |
| ARG81938-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81938-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81938-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81938-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81938-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81938-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81938-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81938-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81938-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81938 Human SPHK1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human SPHK1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | SPHK1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 156 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 312 - 20000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.8%; Inter-Assay CV: 6.7% |
| 別名 | SK 1; SPK 1; EC 2.7.1.91; SPHK; Sphingosine kinase 1 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SPHK1 |
| 全名 | sphingosine kinase 1 |
| 背景介绍 | The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| 生物功能 | Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. [UniProt] |
| 产品亮点 | Related products: SPHK1 antibodies; SPHK1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1) Click the Picture to Zoom In
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ARG81938 Human SPHK1 ELISA Kit standard curve image
ARG81938 Human SPHK1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81938 Human SPHK1 ELISA Kit User's manual |
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