ARG82385
Human Robo1 / DUTT1 ELISA Kit
Human Robo1 / DUTT1 ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82385-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82385-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82385-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82385-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82385-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82385-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82385-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82385-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82385-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82385-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82385-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82385 Human Robo1 / DUTT1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Robo1 / DUTT1 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | Robo1 / DUTT1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 31.25 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| 标准范围 | 62.5 - 4000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 4.9% Inter-Assay CV: 6.5% |
| 別名 | Deleted in U twenty twenty; H-Robo-1; DUTT1; Roundabout homolog 1; SAX3 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ROBO1 |
| 全名 | roundabout guidance receptor 1 |
| 背景介绍 | Bilateral symmetric nervous systems have special midline structures that establish a partition between the two mirror image halves. Some axons project toward and across the midline in response to long-range chemoattractants emanating from the midline. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| 生物功能 | Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. Cell projection, axon. Note=Detected at growth cones in thalamus neurons. [UniProt] |
| 产品亮点 | Related products: Robo1 antibodies; Robo1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Ubiquitinated. May be deubiquitinated by USP33. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG82385 Human Robo1 / DUTT1 ELISA Kit standard curve image
ARG82385 Human Robo1 / DUTT1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82385 Human Robo1 / DUTT1 ELISA Kit User manual |
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