ARG70238
Human RSPO1 / R spondin 1 recombinant protein (Active) (His-tagged, C-ter)
Human RSPO1 / R spondin 1 recombinant protein (Active) (His-tagged, C-ter) for Functional study,SDS-PAGE and Human
概述
| 产品描述 | HEK293 expressed, His-tagged (C-ter) Active Human RSPO1 / R spondin 1 recombinant protein. |
|---|---|
| 反应物种 | Hu |
| 应用 | FuncSt, SDS-PAGE |
| 靶点名称 | RSPO1 / R spondin 1 |
| 物种 | Human |
| A.A. 序列 | Arg31 - Ala263 of Human RSPO1 / R spondin 1 (NP_001033722.1) with 6X His tag at the C - terminus. |
| 表达系统 | HEK293 |
| 生物活性 | Active |
| 活性说明 | Measured by its ability to enhance Cyclin D1 expression in HCT116 human colon adenocarcinoma cells. 0.1-10 ng/ml of Recombinant Human RSPO1 can effectively enhance Cyclin D1 expression. |
| 別名 | hRspo1; Roof plate-specific spondin-1; R-spondin-1; RSPO; CRISTIN3 |
属性
| 形式 | Powder |
|---|---|
| 纯化说明 | 0.22 µm filter sterilized. Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 92% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 复溶 | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | RSPO1 |
|---|---|
| 全名 | R-spondin 1 |
| 背景介绍 | This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] |
| 生物功能 | Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors (PubMed:29769720). Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Regulates Wnt signaling by antagonizing DKK1/KREM1-mediated internalization of LRP6 through an interaction with KREM1 (PubMed:17804805). [UniProt] |
| 细胞定位 | Secreted. Nucleus. Note=Seems to mainly localize to nucleoli. [UniProt] |
| 预测分子量 | 29 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG70238 Human RSPO1 / R spondin 1 recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70238 Human RSPO1 / R spondin 1 recombinant protein (Active) (His-tagged, C-ter).
