ARG80173

Human Perforin ELISA Kit

Human Perforin ELISA 试剂盒 for ELISA and Human

Immune System kit
publication_link 参考文献6

内含物

货号 内含物名称 包装 温度
ARG80173-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80173-002 Standard (Lyophilized) 2 X 2.5 ng/vial 4°C
ARG80173-003 Standard diluent buffer 20 ml 4°C
ARG80173-004 Antibody conjugate concentrate 400 µl 4°C
ARG80173-005 Antibody diluent buffer 16 ml 4°C
ARG80173-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80173-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80173-008 20X Wash buffer 50 ml 4°C
ARG80173-009 TMB substrate 12ml 4°C (Protect from light)
ARG80173-010 STOP solution 12ml 4°C
ARG80173-011 Plate sealer 4 strips Room temperature

概述

产品描述 ARG80173 Human Perforin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Perforin in Serum, Plasma, Cell culture supernatants.
反应物种 Hu
应用 ELISA
特异性 No significant cross-reactivity or interference with Human IL-1 beta, IL-2, IL-3, IFN- gamma, TNF alpha, Fas, Fas-L, TRAIL
靶点名称 Perforin
偶联标记 HRP
偶联标记说明

Substrate: TMB and read at 450 nm

灵敏度 20 pg/ml
样品类型 Serum, Plasma, Cell culture supernatants
标准范围 39 - 2500 pg/ml
样本量 100 µl
精确

CV: <10%

別名 FLH2; P1; PFN1; Cytolysin; HPLH2; PFP; Lymphocyte pore-forming protein; Perforin-1

应用说明

检测时间 4 hours

属性

形式 96 well
存放说明 Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 5551 Human PRF1

Swiss-port # P14222 Human Perforin-1

基因名称 PRF1
全名 perforin 1 (pore forming protein)
背景介绍 Perforin with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017]
生物功能 Perforin plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. [UniProt]
产品亮点 Related products:
Perforin antibodies; Perforin ELISA Kits;
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研究领域 Immune System kit
翻译后修饰 N-glycosylated.

检测图片 (1) Click the Picture to Zoom In

  • ARG80173 Human Perforin ELISA Kit standard curve image

    ARG80173 Human Perforin ELISA Kit results of a typical standard run with optical density reading at 450 nm.

文献引用

Redirecting natural killer cells to potentiate adoptive immunotherapy in solid tumors through stabilized Y-type bispecific aptamer

ELISA / Human / Cell culture supernatants

Youshi Zheng  et al.
Nanoscale.,  (2021)

publication_link

 

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Acute lymphoblastic leukemia-derived exosome inhibits cytotoxicity of natural killer cells by TGF-β signaling pathway

ELISA / Human / Cell culture supernatants

Huijun Yu et al.
3 Biotech.,  (2021)

publication_link

 

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B7-H3 inhibits the IFN-γ-dependent cytotoxicity of Vγ9Vδ2 T cells against colon cancer cells.

ELISA / Human / Cell culture supernatant

Lu Huimin et al.
OncoImmunology.,  (2020)

publication_link

 

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Tim-3 suppresses the killing effect of Vγ9Vδ2 T cells on colon cancer cells by reducing perforin and granzyme B expression.

ELISA / Human

Li Xiaomi et al.
Exp Cell Res.,  (2019)

publication_link

 

hr_line

The Effect of Tea Catechins on Natural Killer Cell Activity in the Elderly: A Pilot Study.

ELISA / Human

Iketani Ryo et al.
Japanese Journal of Clinical Pharmacology and Therapeutics.,  (2019)

publication_link

 

hr_line

Increase of Tumor Infiltrating γδ T-cells in Pancreatic Ductal Adenocarcinoma Through Remodeling of the Extracellular Matrix by a Hyaluronan Synthesis Suppressor, 4-Methylumbelliferone.

ELISA / Human

Suto Akiko et al.
Pancreas.,  (2019)

publication_link

 

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Title Download Link
ARG80173 Human Perforin ELISA Kit User's manual download icon Download