ARG70298
Human PVRL3 recombinant protein (Fc-His-tagged, C-ter)
Human PVRL3 recombinant protein (Fc-His-tagged, C-ter) for Binding Activity,SDS-PAGE and Human
概述
| 产品描述 | HEK293 expressed, Fc-His-tagged (C-ter) Human PVRL3 recombinant protein. |
|---|---|
| 反应物种 | Hu |
| 应用 | Binding, SDS-PAGE |
| 靶点名称 | PVRL3 |
| 物种 | Human |
| A.A. 序列 | Gly58 - Asp400 of Human PVRL3 (NP_056295) with an Fc - 6X His tag at the C - terminus. |
| 表达系统 | HEK293 |
| 別名 | PVRR3; PPR3; CDW113; PRR3; Nectin-3; CDw113; CD113; NECTIN-3; Poliovirus receptor-related protein 3; CD antigen CD113 |
应用说明
| 应用说明 | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Nectin-1 at 3µg/ml (100 µl/well) can bind Recombinant Human Nectin-3 with a linear range of 180-720 ng/ml. |
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属性
| 形式 | Powder |
|---|---|
| 纯化说明 | 0.22 µm filter sterilized. Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 97% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 复溶 | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | PVRL3 |
|---|---|
| 全名 | poliovirus receptor-related 3 |
| 背景介绍 | This gene encodes a member of the nectin family of proteins, which function as adhesion molecules at adherens junctions. This family member interacts with other nectin-like proteins and with afadin, a filamentous actin-binding protein involved in the regulation of directional motility, cell proliferation and survival. This gene plays a role in ocular development involving the ciliary body. Mutations in this gene are believed to result in congenital ocular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011] |
| 生物功能 | Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with NECTIN2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane. [UniProt] |
| 预测分子量 | 61 kDa |
检测图片 (1) Click the Picture to Zoom In
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ARG70298 Human PVRL3 recombinant protein (ECD) (Fc-His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70298 Human PVRL3 recombinant protein (ECD) (Fc-His-tagged, C-ter).
