ARG80166
Human PDGF BB ELISA Kit
Human PDGF BB ELISA 试剂盒 for ELISA and Human
Cancer kit; Cell Biology and Cellular Response kit; Developmental Biology kit; Metabolism kit; Signaling Transduction kit
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG80166-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80166-002 | Standard (Lyophilized) | 3 X 4 ng/vial | 4°C |
| ARG80166-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80166-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80166-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80166-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80166-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80166-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80166-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80166-010 | STOP solution | 12ml | 4°C |
| ARG80166-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG80166 Human PDGF BB ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human PDGF BB in serum, plasma and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | No significant cross-reactivity or interference with Human PDGF R alpha |
| 靶点名称 | PDGF BB |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma and cell culture supernatants. |
| 标准范围 | 31.25 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | CV: less than10% |
| 別名 | SIS; Becaplermin; Platelet-derived growth factor subunit B; IBGC5; PDGF subunit B; c-sis; SSV; PDGF-2; Proto-oncogene c-Sis; Platelet-derived growth factor beta polypeptide; PDGF2; Platelet-derived growth factor B chain |
应用说明
| 检测时间 | 4 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P01127 Human Platelet-derived growth factor subunit B |
|---|---|
| 基因名称 | PDGFB |
| 全名 | platelet-derived growth factor beta polypeptide |
| 背景介绍 | The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq] |
| 产品亮点 | Related products: PDGF BB antibodies; PDGF BB ELISA Kits; Related news: Vascular development is regulated by FGF-dependent metabolic control The role of HDGF in tumor angiogenesis New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 研究领域 | Cancer kit; Cell Biology and Cellular Response kit; Developmental Biology kit; Metabolism kit; Signaling Transduction kit |
检测图片 (1) Click the Picture to Zoom In
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ARG80166 Human PDGF BB ELISA Kit standard curve image
ARG80166 Human PDGF BB ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80166 Human PDGF-BB ELISA Kit User's manual |
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