ARG82498
Human PAM / PAL ELISA Kit
Human PAM / PAL ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82498-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82498-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82498-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82498-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82498-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82498-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82498-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82498-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82498-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82498-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82498-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82498 Human PAM / PAL ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human PAM / PAL in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | PAM / PAL |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 156 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| 标准范围 | 312 - 20000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 4.8% Inter-Assay CV: 6.7% |
| 別名 | Peptidylamidoglycolate lyase; PHM; EC 4.3.2.5; Peptidyl-glycine alpha-amidating monooxygenase; EC 1.14.17.3; PAL; PAM |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # P19021 Human Peptidyl-glycine alpha-amidating monooxygenase |
|---|---|
| 基因名称 | PAM |
| 全名 | peptidylglycine alpha-amidating monooxygenase |
| 背景介绍 | This gene encodes a multifunctional protein. It has two enzymatically active domains with catalytic activities - peptidylglycine alpha-hydroxylating monooxygenase (PHM) and peptidyl-alpha-hydroxyglycine alpha-amidating lyase (PAL). These catalytic domains work sequentially to catalyze neuroendocrine peptides to active alpha-amidated products. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene but some of their full length sequences are not yet known. [provided by RefSeq, Jul 2008] |
| 生物功能 | Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity. [UniProt] |
| 细胞定位 | Isoform 1: Membrane; Single-pass type I membrane protein. Isoform 2: Membrane; Single-pass type I membrane protein. Isoform 3: Secreted. Note=Secreted from secretory granules. Isoform 4: Secreted. Note=Secreted from secretory granules. [UniProt] |
| 产品亮点 | Related products: PAM / PAL antibodies; PAM / PAL ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1) Click the Picture to Zoom In
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ARG82498 Human PAM / PAL ELISA Kit standard curve image
ARG82498 Human PAM / PAL ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82498 Human PAM / PAL ELISA Kit User manual |
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