ARG82466
Human Nephrin ELISA Kit
Human Nephrin ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82466-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82466-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82466-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82466-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82466-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82466-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82466-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82466-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82466-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82466-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82466-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82466 Human Nephrin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Nephrin in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | Nephrin |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 4.4% Inter-Assay CV: 6.3% |
| 別名 | CNF; NPHN; Renal glomerulus-specific cell adhesion receptor; nephrin; Nephrin |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | NPHS1 |
| 全名 | nephrosis 1, congenital, Finnish type (nephrin) |
| 背景介绍 | This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009] |
| 生物功能 | Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity). [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. Note=Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane. [UniProt] |
| 产品亮点 | Related products: Nephrin antibodies; Nephrin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Phosphorylated at Tyr-1193 by FYN, leading to the recruitment and activation of phospholipase C-gamma-1/PLCG1. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG82466 Human Nephrin ELISA Kit standard curve image
ARG82466 Human Nephrin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82466 Human Nephrin ELISA Kit User manual |
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