ARG82828
Human NRG1 / Heregulin beta 1 ELISA Kit
Human NRG1 / Heregulin beta 1 ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82828-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82828-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82828-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82828-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82828-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82828-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82828-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82828-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82828-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82828-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82828-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82828 Human NRG1 / Heregulin beta 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human NRG1 / Heregulin beta 1 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | NRG1 / Heregulin beta 1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 31.3 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 62.5 - 4000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.6% Inter-Assay CV: 6.9% |
| 別名 | Sensory and motor neuron-derived factor; Heregulin; GGF2; Glial growth factor; Acetylcholine receptor-inducing activity; SMDF; ARIA; NRG1-IT2; Neu differentiation factor; HRGA; NDF; Breast cancer cell differentiation factor p45; HGL; GGF; MSTP131; Pro-NRG1; HRG; MST131; HRG1; Pro-neuregulin-1, membrane-bound isoform |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q02297 Human Pro-neuregulin-1, membrane-bound isoform |
|---|---|
| 基因名称 | NRG1 |
| 全名 | neuregulin 1 |
| 背景介绍 | The protein encoded by this gene is a membrane glycoprotein that mediates cell-cell signaling and plays a critical role in the growth and development of multiple organ systems. An extraordinary variety of different isoforms are produced from this gene through alternative promoter usage and splicing. These isoforms are expressed in a tissue-specific manner and differ significantly in their structure, and are classified as types I, II, III, IV, V and VI. Dysregulation of this gene has been linked to diseases such as cancer, schizophrenia, and bipolar disorder (BPD). [provided by RefSeq, Apr 2016] |
| 生物功能 | Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. Binds to ERBB4 (PubMed:10867024, PubMed:7902537). Binds to ERBB3 (PubMed:20682778). Acts as a ligand for integrins and binds (via EGF domain) to integrins ITGAV:ITGB3 or ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and ERRB3 are essential for NRG1-ERBB signaling. Induces the phosphorylation and activation of MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed:20682778). Ligand-dependent ERBB4 endocytosis is essential for the NRG1-mediated activation of these kinases in neurons (By similarity). [UniProt] |
| 细胞定位 | Pro-neuregulin-1, membrane-bound isoform: Cell membrane; Single-pass type I membrane protein. Note=Does not seem to be active. Neuregulin-1: Secreted. Isoform 8: Nucleus. Note=May be nuclear. Isoform 9: Secreted. Note=Has a signal peptide. Isoform 10: Membrane; Single-pass type I membrane protein. Note=May possess an internal uncleaved signal sequence. [UniProt] |
| 翻译后修饰 | Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form. N- and O-glycosylated. Extensive glycosylation precedes the proteolytic cleavage (By similarity). [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG82828 Human NRG1 / Heregulin beta 1 ELISA Kit standard curve image
ARG82828 Human NRG1 / Heregulin beta 1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82828 Human NRG1 / Heregulin beta 1 ELISA Kit User manual |
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