ARG81556
Human Mesothelin ELISA Kit
Human Mesothelin ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81556-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81556-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81556-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81556-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81556-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81556-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81556-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81556-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81556-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81556-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81556-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81556 Human Mesothelin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Mesothelin in serum, plasma (heparin, EDTA), saliva, urine and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | Mesothelin |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA), saliva, urine and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.4% Inter-Assay CV: 6.6% |
| 別名 | MPF; Mesothelin; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor; SMRP |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MSLN |
| 全名 | mesothelin |
| 背景介绍 | This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010] |
| 生物功能 | Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. [UniProt] |
| 产品亮点 | Related products: Mesothelin antibodies; Mesothelin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Both MPF and the cleaved form of mesothelin are N-glycosylated. Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG81556 Human Mesothelin ELISA Kit standard curve image
ARG81556 Human Mesothelin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81556 Human Mesothelin ELISA Kit User manual |
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