ARG70276
Human MMP1 recombinant protein (Active) (His-tagged, C-ter)
Human MMP1 recombinant protein (Active) (His-tagged, C-ter) for Functional study,SDS-PAGE and Human
概述
| 产品描述 | HEK293 expressed, His-tagged (C-ter) Active Human MMP1 recombinant protein. |
|---|---|
| 反应物种 | Hu |
| 应用 | FuncSt, SDS-PAGE |
| 靶点名称 | MMP1 |
| 物种 | Human |
| A.A. 序列 | Phe20 - Asn469 of Human MMP1 (NP_002412.1) with 6X His tag at the C - terminus. |
| 表达系统 | HEK293 |
| 生物活性 | Active |
| 活性说明 | Measured in a cell migration assay using A549 cells. 1 ng/ml of Recombinant Human MMP-1 can effectively induce A549 cells migration. |
| 別名 | MMP-1; CLG; Fibroblast collagenase; Matrix metalloproteinase-1; CLGN; EC 3.4.24.7; Interstitial collagenase |
属性
| 形式 | Powder |
|---|---|
| 纯化说明 | 0.22 µm filter sterilized. Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 97% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 复溶 | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | MMP1 |
|---|---|
| 全名 | matrix metallopeptidase 1 |
| 生物功能 | Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X (PubMed:2557822, PubMed:2153297, PubMed:1645757). In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity (PubMed:16807369). [UniProt] |
| 细胞定位 | Secreted, extracellular space, extracellular matrix. [UniProt] |
| 预测分子量 | 54 kDa |
| 翻译后修饰 | Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase. Tyrosine phosphorylated in platelets by PKDCC/VLK. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG70276 Human MMP1 recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70276 Human MMP1 recombinant protein (Active) (His-tagged, C-ter).
