ARG82854
Human Ki-67 ELISA Kit
Human Ki-67 ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82854-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82854-002 | Standard | 2 X 1 ng/vial | 4°C |
| ARG82854-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82854-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82854-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82854-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82854-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82854-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82854-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82854-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82854-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82854 Human Ki-67 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Ki-67 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | Ki-67 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 3.9 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| 标准范围 | 7.8 - 500 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.6% Inter-Assay CV: 6.9% |
| 別名 | Antigen KI-67; MIB-; KIA; MIB-1; PPP1R105 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MKI67 |
| 全名 | marker of proliferation Ki-67 |
| 背景介绍 | This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009] |
| 生物功能 | Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable). [UniProt] |
| 细胞定位 | Chromosome. Nucleus. Nucleus, nucleolus. Note=Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface. Associates with satellite DNA in G1 phase. Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes. Predominantly localized in the G1 phase in the perinucleolar region. [UniProt] |
| 翻译后修饰 | Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG82854 Human Ki-67 ELISA Kit standard curve image
ARG82854 Human Ki-67 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82854 Human Ki-67 ELISA Kit User manual |
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