ARG81513
Human KLK11 ELISA Kit
Human KLK11 ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81513-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81513-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81513-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81513-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81513-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81513-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81513-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81513-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81513-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81513-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81513-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81513 Human KLK11 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human KLK11 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | KLK11 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.2% Inter-Assay CV: 7.3% |
| 別名 | Trypsin-like protease; Kallikrein-11; hK11; PRSS20; EC 3.4.21.-; Serine protease 20; TLSP; Hippostasin |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | KLK11 |
| 全名 | kallikrein-related peptidase 11 |
| 背景介绍 | Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in multiple transcript variants encoding distinct isoforms which are differentially expressed.[provided by RefSeq, Nov 2009] |
| 生物功能 | Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues. [UniProt] |
| 产品亮点 | Related products: KLK antibodies; KLK ELISA Kits; KLK Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | About 40% of KLK11 is inactivated by internal cleavage after Arg-188. This proteolytic inactivation may be effected by plasminogen. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG81513 Human KLK11 ELISA Kit standard curve image
ARG81513 Human KLK11 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81513 Human KLK11 ELISA Kit User's manual |
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