ARG82366
Human ITIH4 ELISA Kit
Human ITIH4 ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82366-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82366-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82366-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82366-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82366-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82366-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82366-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82366-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82366-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82366-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82366-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82366 Human ITIH4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human ITIH4 in serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | ITIH4 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin, citrate) and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 4.8% Inter-Assay CV: 7.0% |
| 別名 | PK-120; PK120; Inter-alpha-inhibitor heavy chain 4; Gp120; H4P; GP120; Inter-alpha-trypsin inhibitor family heavy chain-related protein; Inter-alpha-trypsin inhibitor heavy chain H4; IHRP; ITIHL1; Plasma kallikrein sensitive glycoprotein 120; ITI heavy chain H4; ITI-HC4 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 |
Swiss-port # Q14624 Human Inter-alpha-trypsin inhibitor heavy chain H4 |
|---|---|
| 基因名称 | ITIH4 |
| 全名 | inter-alpha-trypsin inhibitor heavy chain family, member 4 |
| 背景介绍 | The protein encoded by this gene is secreted into the blood, where it is cleaved by plasma kallikrein into two smaller forms. Expression of this gene has been detected only in liver, and it seems to be upregulated during surgical trauma. This gene is part of a cluster of similar genes on chromosome 3. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] |
| 生物功能 | Type II acute-phase protein (APP) involved in inflammatory responses to trauma. May also play a role in liver development or regeneration. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 产品亮点 | Related products: ITIH4 antibodies; ITIH4 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Cleaved by plasma kallikrein to yield 100 kDa and 35 kDa fragments, and the resulting 100 kDa fragment is further converted to a 70 kDa fragment. N- and O-glycosylated. In urine, O-linked glycosylation on threonine residues in the region from Thr-719 to Thr-725 consists of core 1 or possibly core 8 glycans. Mainly Hex(HexNAc)(2), but also some Hex(3)(HexNAc)(3). N-glycosylated but not O-glycosylated in plasma. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG82366 Human ITIH4 ELISA Kit standard curve image
ARG82366 Human ITIH4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG82366 Human ITIH4 ELISA Kit User manual |
 Download
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