ARG70031

Human IL8 recombinant protein (Active) (His-tagged, C-ter)

Human IL8 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE

概述

产品描述 E. coli expressed, His-tagged (C-ter) Active Human IL8 recombinant protein
应用 SDS-PAGE
靶点名称 IL8
物种 Human
A.A. 序列 Ser28 - Ser99
表达系统 E. coli
生物活性 Active
活性说明 Determined by its ability to chemoattract PBMC. The ED50 for this effect is <2 ng/mL.
別名 IL8/NAP1 form IV; GCP/IL-8 protein IV; NAF; T-cell chemotactic factor; 1-77; Ala-IL-8; Interleukin-8; IL-8; Neutrophil-activating protein 1; GCP/IL-8 protein II; IL8/NAP1 form II; GCP/IL-8 protein V; MDNCF; Protein 3-10C; Lymphocyte-derived neutrophil-activating factor; Neutrophil-activating factor; Granulocyte chemotactic protein 1; LYNAP; NAP-1; Monocyte-derived neutrophil chemotactic factor; 6-77; 7-77; C-X-C motif chemokine 8; GCP1; NAP1; Ser-IL-8; 5-77; GCP/IL-8 protein VI; IL8/NAP1 form I; IL8/NAP1 form VI; Monocyte-derived neutrophil-activating peptide; C-X-C motif; 8-77; 9-77; LUCT; Chemokine; GCP-1; MDNCF-b; MDNCF-c; IL8/NAP1 form V; LECT; IL8/NAP1 form III; GCP/IL-8 protein III; Emoctakin; GCP/IL-8 protein I; MONAP; IL8

属性

形式 Powder
纯化说明 Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test.
纯度 >98% (by SDS-PAGE)
缓冲液 PBS (pH 8.0)
复溶 It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely.
存放说明 For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

For laboratory research only, not for drug, diagnostic or other use.

基因名称 CXCL8
全名 chemokine (C-X-C motif) ligand 8
背景介绍 The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. [provided by RefSeq, Jul 2008]
生物功能 IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. [UniProt]
细胞定位 Secreted. [UniProt]
产品亮点 Related products:
IL8 antibodies; IL8 ELISA Kits; IL8 Duos / Panels; IL8 recombinant proteins;
Related news:
HMGB1 in inflammation
Inflammatory Cytokines
翻译后修饰 Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.

Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation. [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG70031 Human IL8 recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image

    SDS-PAGE analysis of ARG70031 Human IL8 recombinant protein (Active) (His-tagged, C-ter).