ARG80179

Human IL33 ELISA Kit

Datasheet

参考文献1

订购信息

包装

价格 (USD)

96 wells

439
250

arigo in CHINA:
恩瑞科生物
☎ 400-886-0853,
0571-8708-1890

内含物

货号	内含物名称	包装	温度
ARG80179-001	Antibody-coated microplate	8 X 12 strips	4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80179-002	Standard (Lyophilized)	3 X 4 ng/vial	4°C
ARG80179-003	Standard diluent buffer	20 ml	4°C
ARG80179-004	Antibody conjugate concentrate	400 µl	4°C
ARG80179-005	Antibody diluent buffer	16 ml	4°C
ARG80179-006	HRP-Streptavidin concentrate	400 µl	4°C (Protect from light)
ARG80179-007	HRP-Streptavidin diluent buffer	16 ml	4°C
ARG80179-008	20X Wash buffer	50 ml	4°C
ARG80179-009	TMB substrate	12ml	4°C (Protect from light)
ARG80179-010	STOP solution	12ml	4°C
ARG80179-011	Plate sealer	4 strips	Room temperature

概述

产品描述	ARG80179 Human IL33 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL33 in Serum, Plasma, Cell culture supernatants.
反应物种	Hu
应用	ELISA
特异性	No significant cross-reactivity or interference with Human IL-1α, IL-1β, IL-18, IL-36α,IL-36β, IL-37
靶点名称	IL33
偶联标记	HRP
偶联标记说明	Substrate: TMB and read at 450 nm
灵敏度	8 pg/ml
样品类型	Serum, Plasma, Cell culture supernatants
标准范围	15.6 - 1000 pg/ml
样本量	100 µl
精确	CV: less than10%
別名	95-270; NF-HEV; Interleukin-33; C9orf26; IL1F11; 99-270; Interleukin-1 family member 11; IL-33; IL-1F11; Nuclear factor from high endothelial venules; NFEHEV; DVS27; 109-270

应用说明

检测时间	4 hours

属性

形式	96 well
存放说明	Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 90865 Human IL33 Swiss-port # O95760 Human Interleukin-33
基因名称	IL33
全名	interleukin 33
背景介绍	Interleukin-33 (IL-33) is a recently identified member of the IL-1 family of cytokines whose other members include IL-1a/b, IL-1Ra and IL-18. Its receptor has been shown to be ST2, an IL-1 receptor family member that also acts as a negative regulator of TLR-IL-1R signaling (1,3) and IL-1R accessory protein (IL-1RAcP). Receptor binding of IL-33 activates NF-kB and MAP kinases and induces the expression of TH2-associated cytokines such as IL-4, IL-5 and IL-6. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties.
产品亮点	Related products: IL33 antibodies; IL33 ELISA Kits; IL33 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData
研究领域	Cell Biology and Cellular Response kit; Immune System kit
翻译后修饰	The full length protein can be released from cells and is able to signal via the IL1RL1/ST2 receptor. However, proteolytic processing by CSTG/cathepsin G and ELANE/neutrophil elastase produces C-terminal peptides that are more active than the unprocessed full length protein. May also be proteolytically processed by calpains (PubMed:19596270). Proteolytic cleavage mediated by apoptotic caspases including CASP3 and CASP7 results in IL33 inactivation (PubMed:19559631). In vitro proteolytic cleavage by CASP1 was reported (PubMed:16286016) but could not be confirmed in vivo (PubMed:19465481) suggesting that IL33 is probably not a direct substrate for that caspase.

检测图片 (1) Click the Picture to Zoom In

ARG80179 Human IL33 ELISA Kit standard curve image

ARG80179 Human IL33 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

文献引用

Interleukin-33 increases the sensitivity of multiple myeloma cells to the proteasome inhibitor bortezomib through reactive oxygen species-mediated inhibition of nuclear factor kappa-B signal and stemness properties

ELISA / Human/Plasma

Ruonan Shao et al.
MedComm (2020), (2024)