ARG80828
Human IL10 ELISA Kit
Human IL10 ELISA 试剂盒 for ELISA and Human
Cancer kit; Immune System kit
概述
| 产品描述 | ARG80828 Human IL10 ELISA Kit is an enzyme immunoassay kit for in vitro quantitative measurement of human interleukin-10 (IL-10) in serum. |
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| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | IL10 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm |
| 灵敏度 | 1.6 pg/ml |
| 样品类型 | Serum. |
| 标准范围 | 14 - 2,101 pg/ml |
| 样本量 | 100 µl |
| 別名 | IL10A; GVHDS; TGIF; IL-10; Cytokine synthesis inhibitory factor; CSIF; Interleukin-10 |
应用说明
| 检测时间 | 2 h, 2 h, 30 min (RT/shaking) |
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属性
| 形式 | 96 well |
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| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
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| 基因名称 | IL10 |
| 全名 | interleukin 10 |
| 背景介绍 | Biological activities Human interleukin-10 (IL-10) is a 19 kDA lymphokine produced by T helper lymphocytes,by monocytes, macrophages and B-lymphocytes. IL-10 was first characterized as a cytokine synthesis inhibitory factor (CSIF) able to inhibit cytokine synthesis by TH1 clones activated in the presence of antigen presenting cells. However, in the absence of monocytes, IL-10 directly inhibits the growth of T-cells triggered by immobilized anti-CD3 MoAb. This proliferation inhibition was found to be a result of specific inhibition of IL-2 production by the responding T-cells. In vitro, Il-10 is a very powerful inhibitor of monokines (including TNF-alpha, IL-1, IL-6 and IL-8) produced by LPS-activated monocytes and macrophages. The addition of IL-10 to B lymphocytes results in limited cell proliferation but most importantly in very high immunoglobulin production, a result of the transformation of B-cells into plasma cells. Finally, natural killer (NK) cells appear to be another target for the anti-inflammatory properties of IL-10. Indeed, recent data have shown that IL-10 can inhibit antigen induced IFN-gamma production by NK-cells by inhibiting not only production but also the stimulatory effects of IL-12 and TNF on IFN-gamma production. Clinical application So far, circulating levels of IL-10 have been found in serum of patients suffering of Non-Hodgkin's lymphoma, multiple myeloma, cerebral malaria or septic shock. |
| 研究领域 | Cancer kit; Immune System kit |
检测图片 (1) Click the Picture to Zoom In
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ARG80828 Human IL10 ELISA Kit example of standard curve image
ARG80828 Human IL10 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG80828 Human IL10 ELISA Kit User's manual |
 Download
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