ARG70035
Human IFN gamma recombinant protein (Active) (His-tagged, C-ter)
Human IFN gamma recombinant protein (Active) (His-tagged, C-ter) for Functional study,SDS-PAGE and Human
概述
| 产品描述 | E. coli expressed, His-tagged (C-ter) Active Human IFN gamma recombinant protein |
|---|---|
| 反应物种 | Hu |
| 应用 | FuncSt, SDS-PAGE |
| 靶点名称 | IFN gamma recombinant protein |
| 物种 | Human |
| A.A. 序列 | MQDPY VKEAE NLKKY FNAGH SDVAD NGTLF LGILK NWKEE SDRKI MQSQI VSFYF KLFKN FKDDQ SIQKS VETIK EDMNV KFFNS NKKKR DDFEK LTNYS VTDLN VQRKA IHELI QVMAE LSPAA KTGKR KRSQM LFQGR RASQ with polyhistidine tag at the C-terminus. |
| 表达系统 | E. coli |
| 生物活性 | Active |
| 活性说明 | Determined by its ability to induce cytotoxicity in HT29 cells. The ED50 for this effect is < 1.1 ng/ml. |
| 別名 | IFN-gamma; Interferon gamma; Immune interferon; IFG; IFI |
属性
| 形式 | Powder |
|---|---|
| 纯化 | Ni-NTA chromatography. |
| 纯化说明 | Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 8.0) |
| 复溶 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 100 µg/ml and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| 存放说明 | Lyophilized protein should be stored at -20°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | IFNG |
| 全名 | interferon, gamma |
| 背景介绍 | This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of the both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial inflections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Sep 2015] |
| 生物功能 | Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. [UniProt] |
| 细胞定位 | Secreted. [UniProt] |
| 产品亮点 | Related products: IFN gamma antibodies; IFN gamma ELISA Kits; IFN gamma Duos / Panels; IFN gamma recombinant proteins; Related news: HMGB1 in inflammation Inflammatory Cytokines |
| 翻译后修饰 | Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG70035 Human IFN gamma recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70035 Human IFN gamma recombinant protein (Active) (His-tagged, C-ter).
