ARG81772
Human Heme Oxygenase 1 ELISA Kit
Human Heme Oxygenase 1 ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81772-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81772-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81772-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81772-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81772-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81772-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81772-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81772-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81772-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81772-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81772-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81772 Human Heme Oxygenase 1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Heme Oxygenase 1 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | Heme Oxygenase 1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 46.85 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 93.7 - 6000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.9%; Inter-Assay CV: 7.2% |
| 別名 | bK286B10; Heme oxygenase 1; HO-1; EC 1.14.99.3; HMOX1D; HSP32 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | HMOX1 |
| 全名 | heme oxygenase 1 |
| 背景介绍 | Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq, Jul 2008] |
| 生物功能 | Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis. [UniProt] |
| 产品亮点 | Related products: Heme Oxygenase 1 antibodies; Heme Oxygenase 1 ELISA Kits; Heme Oxygenase 1 Duos / Panels; Related news: Keap1-Nrf2-ARE antibody panel is launched New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1) Click the Picture to Zoom In
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ARG81772 Human Heme Oxygenase 1 ELISA Kit standard curve image
ARG81772 Human Heme Oxygenase 1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81772 Human Heme Oxygenase 1 ELISA Kit User's manual |
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