ARG70237
Human HVEM / TR2 recombinant protein (Fc-His-tagged, C-ter)
Human HVEM / TR2 recombinant protein (Fc-His-tagged, C-ter) for Binding Activity,SDS-PAGE and Human
概述
| 产品描述 | HEK293 expressed, Fc-His-tagged (C-ter) Human HVEM / TR2 recombinant protein. |
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| 反应物种 | Hu |
| 应用 | Binding, SDS-PAGE |
| 靶点名称 | HVEM / TR2 |
| 物种 | Human |
| A.A. 序列 | Leu39 - Val202 of Human HVEM / TR2 (NP_003811.2) with an Fc - 6X His tag at the C - terminus. |
| 表达系统 | HEK293 |
| 別名 | Herpes virus entry mediator A; HVEM; Tumor necrosis factor receptor-like 2; HVEA; CD antigen CD270; CD270; Tumor necrosis factor receptor superfamily member 14; LIGHTR; HveA; Herpesvirus entry mediator A; ATAR; TR2 |
应用说明
| 应用说明 | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant human HVEM at 5 µg/ml (100 µl/well) can bind Biotinylated Recombinant human BTLA with a linear range of 1.5-6 ug/ml. |
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属性
| 形式 | Powder |
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| 纯化说明 | 0.22 µm filter sterilized. Endotoxin level is <0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 97% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 复溶 | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | TNFRSF14 |
|---|---|
| 全名 | tumor necrosis factor receptor superfamily, member 14 |
| 背景介绍 | This gene encodes a member of the TNF (tumor necrosis factor) receptor superfamily. The encoded protein functions in signal transduction pathways that activate inflammatory and inhibitory T-cell immune response. It binds herpes simplex virus (HSV) viral envelope glycoprotein D (gD), mediating its entry into cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| 生物功能 | Receptor for four distinct ligands: The TNF superfamily members TNFSF14/LIGHT and homotrimeric LTA/lymphotoxin-alpha and the immunoglobulin superfamily members BTLA and CD160, altogether defining a complex stimulatory and inhibitory signaling network (PubMed:9462508, PubMed:10754304, PubMed:18193050, PubMed:23761635). Signals via the TRAF2-TRAF3 E3 ligase pathway to promote immune cell survival and differentiation (PubMed:19915044, PubMed:9153189, PubMed:9162022). Participates in bidirectional cell-cell contact signaling between antigen presenting cells and lymphocytes. In response to ligation of TNFSF14/LIGHT, delivers costimulatory signals to T cells, promoting cell proliferation and effector functions (PubMed:10754304). Interacts with CD160 on NK cells, enhancing IFNG production and anti-tumor immune response (PubMed:23761635). In the context of bacterial infection, acts as a signaling receptor on epithelial cells for CD160 from intraepithelial lymphocytes, triggering the production of antimicrobial proteins and proinflammatory cytokines (By similarity). Upon binding to CD160 on activated CD4+ T cells, downregulates CD28 costimulatory signaling, restricting memory and alloantigen-specific immune response (PubMed:18193050). May interact in cis (on the same cell) or in trans (on other cells) with BTLA (PubMed:19915044) (By similarity). In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells (PubMed:19915044) (By similarity). (Microbial infection) Acts as a receptor for Herpes simplex virus 1/HHV-1. (Microbial infection) Acts as a receptor for Herpes simplex virus 2/HHV-2. [UniProt] |
| 细胞定位 | Membrane; Single-pass type I membrane protein. [UniProt] |
| 预测分子量 | 30 kDa |
| 翻译后修饰 | N-glycosylated. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG70237 Human HVEM / TR2 recombinant protein (ECD) (Fc-His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70237 Human HVEM / TR2 recombinant protein (ECD) (Fc-His-tagged, C-ter).
