ARG70228
Human HGF recombinant protein (Active)
Human HGF recombinant protein (Active) for SDS-PAGE and Human,Monkey
概述
| 产品描述 | Hi-5 insect cells (BTI-Tn-5B1-4) expressed, Active Human HGF recombinant protein |
|---|---|
| 反应物种 | Hu, Mk |
| 应用 | SDS-PAGE |
| 靶点名称 | HGF |
| 物种 | Human |
| A.A. 序列 | Alpha chain: QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG LMDHTESGKI CQRWDHQTPH RHKFLPERYP DKGFDDNYCR NPDGQPRPWC YTLDPHTRWE YCAIKTCADN TMNDTDVPLE TTECIQGQGE GYRGTVNTIW NGIPCQRWDS QYPHEHDMTP ENFKCKDLRE NYCRNPDGSE SPWCFTTDPN IRVGYCSQIP NCDMSHGQDC YRGNGKNYMG NLSQTRSGLT CSMWDKNMED LHRHIFWEPD ASKLNENYCR NPDDDAHGPW CYTGNPLIPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLR Beta chain: VVNGIP TRTNIGWMVS LRYRNKHICG GSLIKESWVL TARQCFPSRD LKDYEAWLGI HDVHGRGDEK CKQVLNVSQL VYGPEGSDLV LMKLARPAVL DDFVSTIDLP NYGCTIPEKT SCSVYGWGYT GLINYDGLLR VAHLYIMGNE KCSQHHRGKV TLNESEICAG AEKIGSGPCE GDYGGPLVCE QHKMRMVLGV IVPGRGCAIP NRPGIFVRVA YYAKWIHKII LTYKVPQS |
| 表达系统 | Hi-5 insect cells (BTI-Tn-5B1-4) |
| 生物活性 | Active |
| 活性说明 | Determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells. The expected ED50 for this effect is 1.0 - 20.0 ng/ml. |
| 別名 | HPTA; Scatter factor; F-TCF; Hepatocyte growth factor; DFNB39; Hepatopoietin-A; HGFB; SF |
属性
| 形式 | Powder |
|---|---|
| 纯化说明 | 0.2 µm filter sterilized. Endotoxin level is <0.1 ng/µg of the protein (<1 EU/µg). |
| 纯度 | > 98% by SDS-PAGE gel and HPLC analyses. |
| 缓冲液 | 10 mM Tris (pH 8.0), 100 mM L-Arginine HCl and 200 mM NaCl. |
| 复溶 | Initially reconstitute in water to 0.5 mg/ml. Note: Allow the reconstituted vial to sit at room temperature for 1 hour before use. Store at 2°C to 8°C for up to 1 week or prepare for extended storage. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | HGF |
|---|---|
| 全名 | hepatocyte growth factor (hepapoietin A; scatter factor) |
| 背景介绍 | This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate alpha and beta chains, which form the mature heterodimer. This protein is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. This protein also plays a role in angiogenesis, tumorogenesis, and tissue regeneration. Although the encoded protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Mutations in this gene are associated with nonsyndromic hearing loss. [provided by RefSeq, Nov 2015] |
| 生物功能 | Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. [UniProt] |
| 预测分子量 | 80 kDa |
