ARG81512
Human Granzyme A ELISA Kit
Human Granzyme A ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81512-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81512-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81512-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81512-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81512-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81512-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81512-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81512-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81512-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81512-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81512-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81512 Human Granzyme A ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Granzyme A in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | Granzyme A |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 7.8 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 15.6 - 1000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.8% Inter-Assay CV: 6.9% |
| 別名 | EC 3.4.21.78; in-1; CTLA3; CTL tryptase; Granzyme A; Hanukkah factor; Cytotoxic T-lymphocyte proteinase 1; H factor; HFSP; HF; Granzyme-1 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | GZMA |
| 全名 | granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3) |
| 背景介绍 | Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells. [provided by RefSeq, Jul 2008] |
| 生物功能 | Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent cell death with morphological features of apoptosis when delivered into the target cell through the immunological synapse. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA. [UniProt] |
| 产品亮点 | Related products: Granzyme antibodies; Granzyme ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1) Click the Picture to Zoom In
-
ARG81512 Human Granzyme A ELISA Kit standard curve image
ARG81512 Human Granzyme A ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81512 Human Granzyme A ELISA Kit User's manual |
 Download
|
