ARG81543
Human Granulysin ELISA Kit
Human Granulysin ELISA 试剂盒 for ELISA and Human
参考文献1
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81543-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81543-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81543-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81543-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81543-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81543-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81543-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81543-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81543-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81543-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81543-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81543 Human Granulysin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Granulysin in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | Granulysin |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.3% Inter-Assay CV: 6.6% |
| 別名 | Lymphokine LAG-2; LAG-2; Granulysin; LAG2; Protein NKG5; T-cell activation protein 519; TLA519; D2S69E; NKG5 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | GNLY |
| 全名 | granulysin |
| 背景介绍 | The product of this gene is a member of the saposin-like protein (SAPLIP) family and is located in the cytotoxic granules of T cells, which are released upon antigen stimulation. This protein is present in cytotoxic granules of cytotoxic T lymphocytes and natural killer cells, and it has antimicrobial activity against M. tuberculosis and other organisms. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| 生物功能 | Antimicrobial protein that kills intracellular pathogens. Active against a broad range of microbes, including Gram-positive and Gram-negative bacteria, fungi, and parasites. Kills Mycobacterium tuberculosis. [UniProt] |
| 产品亮点 | Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | A 9 kDa form is produced by proteolytic processing of a 15 kDa protein. [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG81543 Human Granulysin ELISA Kit standard curve image
ARG81543 Human Granulysin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
文献引用
Granulysin-mediated reduction of PDZRN3 induces Cx43 gap junctions activity exacerbating skin damage in trichloroethylene hypersensitivity syndrome
ELISA / Cell culture supernatants / Human
| Title | Download Link |
|---|---|
| ARG81543 Human Granulysin ELISA Kit User's manual |
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