ARG70599
Human Fibronectin recombinant protein (Active) (His-tagged, C-ter)
Human Fibronectin recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
概述
| 产品描述 | E. coli expressed, His-tagged (C-ter) Active Human Fibronectin recombinant protein |
|---|---|
| 应用 | SDS-PAGE |
| 靶点名称 | Fibronectin |
| 物种 | Human |
| 表达系统 | E. coli |
| 生物活性 | Active |
| 活性说明 | Determined by the ability of the immobilized protein to support the adhesion of B16‑F1 mouse melanoma cells. The ED50 for this effect is less than 100 ng/mL. |
| 別名 | Fibronectin 1; FN; FN1; CIG; FINC; FNZ; MSF; GFND; GFND2; LETS |
属性
| 形式 | Powder |
|---|---|
| 纯化说明 | Endotoxin level is<0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | 50 mM Tris and 150 mM NaCl (pH 8.0) |
| 复溶 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | FN1 |
|---|---|
| 全名 | Fibronectin 1 |
| 背景介绍 | This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined. [provided by RefSeq, Jan 2016] |
| 生物功能 | Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. [UniProt] |
| 细胞定位 | Extracellular matrix; Secreted [UniProt] |
| 翻译后修饰 | Disulfide bond; Glycoprotein; Isopeptide bond; Oxidation; Phosphoprotein; Pyrrolidone carboxylic acid; Sulfation. [UniProt] |
