ARG81134
Human Factor XIII ELISA Kit
Human Factor XIII ELISA 试剂盒 for ELISA and Human
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81134-001 | Antibody-coated Microtiter Plate | 12 x 8 strips | 4°C |
| ARG81134-002 | 20X wash buffer concentrate | 30ml X 2 bottles | 4°C |
| ARG81134-003 | Plate sealer | 3 pieces | 4°C |
| ARG81134-004 | Biotinylated Human Factor XIII antibody (50X) | 1 vial (140 μl) | 4°C |
| ARG81134-005 | Human FXIII Standard | 1 vial (160ng, lyophilized) | 4°C |
| ARG81134-006 | 10X EIA Diluent | 30 ml | 4°C |
| ARG81134-007 | 100X Streptavidin-HRP | 80 μl | 4°C |
| ARG81134-008 | TMB Substrate | 8 ml | 4°C, ready for use |
| ARG81134-009 | STOP solution | 12 ml | 4°C, ready for use |
概述
| 产品描述 | ARG81134 Human Factor XIII ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Factor XIII in serum, plasma, cell culture, urine, saliva, milk and CSF. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | Factor XIII |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm |
| 灵敏度 | 2.1 ng/ml |
| 样品类型 | Serum, plasma, cell culture, urine, saliva, milk and CSF |
| 标准范围 | 2.5 - 160 ng/ml |
| 样本量 | 50 µl |
| 精确 | Intra-Assay CV: 4.4% Inter-Assay CV: 9.4% |
| 別名 | Coagulation factor XIIIa; F13A; Protein-glutamine gamma-glutamyltransferase A chain; Coagulation factor XIII A chain; Transglutaminase A chain; EC 2.3.2.13 |
应用说明
| 检测时间 | ~ 4 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | F13A1 |
| 全名 | coagulation factor XIII, A1 polypeptide |
| 背景介绍 | This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. [provided by RefSeq, Jul 2008] |
| 生物功能 | Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. [UniProt] |
| 产品亮点 | Related products: Factor XIII antibodies; Factor XIII ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | The activation peptide is released by thrombin. |
检测图片 (1) Click the Picture to Zoom In
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ARG81134 Human Factor XIII ELISA Kit standard curve image
ARG81134 Human Factor XIII ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG81134 Human Factor XIII ELISA Kit User's manual |
 Download
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