ARG70129
Human FGF23 recombinant protein (Active) (His-tagged, C-ter)
Human FGF23 recombinant protein (Active) (His-tagged, C-ter) for SDS-PAGE
概述
| 产品描述 | E. coli expressed, His-tagged (C-ter) Active Human FGF23 recombinant protein |
|---|---|
| 应用 | SDS-PAGE |
| 靶点名称 | FGF23 |
| 物种 | Human |
| A.A. 序列 | Tyr25 - Ile251 |
| 表达系统 | E. coli |
| 生物活性 | Active |
| 活性说明 | Determined by its ability to induce proliferation in BaF3 cells transfected with human FGFRIIIc. The ED50 for this effect is < 0.3 μg/mL. |
| 別名 | ADHR; Phosphatonin; HPDR2; FGFN; Tumor-derived hypophosphatemia-inducing factor; PHPTC; FGF-23; Fibroblast growth factor 23; HYPF |
属性
| 形式 | Powder |
|---|---|
| 纯化说明 | Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 98% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 8.0) |
| 复溶 | It is recommended to reconstitute the lyophilized protein in sterile water to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min at room temperature to make sure the protein is dissolved completely. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C or -80°C for up to one month. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | FGF23 |
|---|---|
| 全名 | fibroblast growth factor 23 |
| 背景介绍 | This gene encodes a member of the fibroblast growth factor family of proteins, which possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. The product of this gene regulates phosphate homeostasis and transport in the kidney. The full-length, functional protein may be deactivated via cleavage into N-terminal and C-terminal chains. Mutation of this cleavage site causes autosomal dominant hypophosphatemic rickets (ADHR). Mutations in this gene are also associated with hyperphosphatemic familial tumoral calcinosis (HFTC). [provided by RefSeq, Feb 2013] |
| 生物功能 | Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Upregulates EGR1 expression in the presence of KL (By similarity). Acts directly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization. [UniProt] |
| 细胞定位 | Secreted. Note=Secretion is dependent on O-glycosylation. [UniProt] |
| 翻译后修饰 | Following secretion this protein is inactivated by cleavage into a N-terminal fragment and a C-terminal fragment. The processing is effected by proprotein convertases. O-glycosylated by GALT3. Glycosylation is necessary for secretion; it blocks processing by proprotein convertases when the O-glycan is alpha 2,6-sialylated. Competition between proprotein convertase cleavage and block of cleavage by O-glycosylation determines the level of secreted active FGF23. [UniProt] |
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ARG70129 Human FGF23 recombinant protein (Active) (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70129 Human FGF23 recombinant protein (Active) (His-tagged, C-ter).
