ARG80141
Human FGF basic ELISA Kit
Human FGF basic ELISA 试剂盒 for ELISA and Human
Cancer kit; Cell Biology and Cellular Response kit; Developmental Biology kit; Neuroscience kit; Signaling Transduction kit
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG80141-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80141-002 | Standard (Lyophilized) | 3 X 4 ng/vial | 4°C |
| ARG80141-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80141-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80141-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80141-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80141-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80141-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80141-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80141-010 | STOP solution | 12ml | 4°C |
| ARG80141-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG80141 Human FGF basic ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human FGF basic in serum, plasma and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | No significant cross-reactivity or interference with Human FGF-5,6,9,10,16,17,18,19,20,21,23, KGF； mouse FGF-acidic,FGF-9 |
| 靶点名称 | FGF basic |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm |
| 灵敏度 | 65 pg/ml |
| 样品类型 | Serum, plasma and cell culture supernatants. |
| 标准范围 | 125 - 8000 pg/ml |
| 样本量 | 100 µl |
| 精确 | CV: less than10% |
| 別名 | FGF-2; Fibroblast growth factor 2; bFGF; FGFB; Heparin-binding growth factor 2; BFGF; HBGF-2; Basic fibroblast growth factor |
应用说明
| 检测时间 | 4 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | FGF2 |
| 全名 | fibroblast growth factor 2 (basic) |
| 背景介绍 | The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008] |
| 生物功能 | Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. [UniProt] |
| 产品亮点 | Related products: FGF basic antibodies; FGF basic ELISA Kits; FGF basic recombinant proteins; Related news: Vascular development is regulated by FGF-dependent metabolic control The role of HDGF in tumor angiogenesis New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 研究领域 | Cancer kit; Cell Biology and Cellular Response kit; Developmental Biology kit; Neuroscience kit; Signaling Transduction kit |
| 翻译后修饰 | Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion. Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. |
检测图片 (1) Click the Picture to Zoom In
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ARG80141 Human FGF basic ELISA Kit standard curve image
ARG80141 Human FGF basic ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
|---|---|
| ARG80141 Human FGF basic (bFGF) ELISA Kit User's manual |
 Download
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