ARG80141

Human FGF basic ELISA Kit

Human FGF basic ELISA 试剂盒 for ELISA and Human

Cancer kit; Cell Biology and Cellular Response kit; Developmental Biology kit; Neuroscience kit; Signaling Transduction kit

内含物

货号 内含物名称 包装 温度
ARG80141-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG80141-002 Standard (Lyophilized) 3 X 4 ng/vial 4°C
ARG80141-003 Standard diluent buffer 20 ml 4°C
ARG80141-004 Antibody conjugate concentrate 400 µl 4°C
ARG80141-005 Antibody diluent buffer 16 ml 4°C
ARG80141-006 HRP-Streptavidin concentrate 400 µl 4°C (Protect from light)
ARG80141-007 HRP-Streptavidin diluent buffer 16 ml 4°C
ARG80141-008 20X Wash buffer 50 ml 4°C
ARG80141-009 TMB substrate 12ml 4°C (Protect from light)
ARG80141-010 STOP solution 12ml 4°C
ARG80141-011 Plate sealer 4 strips Room temperature

概述

产品描述 ARG80141 Human FGF basic ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human FGF basic in serum, plasma and cell culture supernatants.
反应物种 Hu
应用 ELISA
特异性 No significant cross-reactivity or interference with Human FGF-5,6,9,10,16,17,18,19,20,21,23, KGF; mouse FGF-acidic,FGF-9
靶点名称 FGF basic
偶联标记 HRP
偶联标记说明

Substrate: TMB and read at 450 nm

灵敏度 65 pg/ml
样品类型 Serum, plasma and cell culture supernatants.
标准范围 125 - 8000 pg/ml
样本量 100 µl
精确

CV: <10%

別名 FGF-2; Fibroblast growth factor 2; bFGF; FGFB; Heparin-binding growth factor 2; BFGF; HBGF-2; Basic fibroblast growth factor

应用说明

检测时间 4 hours

属性

形式 96 well
存放说明 Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 2247 Human FGF2

Swiss-port # P09038 Human Fibroblast growth factor 2

基因名称 FGF2
全名 fibroblast growth factor 2 (basic)
背景介绍 The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]
生物功能 Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. [UniProt]
产品亮点 Related products:
FGF basic antibodies; FGF basic ELISA Kits; FGF basic recombinant proteins;
Related news:
Vascular development is regulated by FGF-dependent metabolic control
The role of HDGF in tumor angiogenesis
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
研究领域 Cancer kit; Cell Biology and Cellular Response kit; Developmental Biology kit; Neuroscience kit; Signaling Transduction kit
翻译后修饰 Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.

检测图片 (1) Click the Picture to Zoom In

  • ARG80141 Human FGF basic ELISA Kit standard curve image

    ARG80141 Human FGF basic ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG80141 Human FGF basic (bFGF) ELISA Kit User's manual download icon Download