ARG80839
Human Estradiol ELISA Kit
Human Estradiol ELISA 试剂盒 for ELISA and Human
Neuroscience kit
概述
| 产品描述 | ARG80839 Human Estradiol ELISA Kit is an Enzyme Immunoassay kit for the quantification of Estradiol in serum and plasma (EDTA). |
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| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | Estradiol |
| 偶联标记 | HRP |
| 灵敏度 | 10.6 pg/ml |
| 样品类型 | Serum and plasma (EDTA, Citrate, heparin). |
| 标准范围 | 25 - 2,000 pg/ml |
| 样本量 | 25 µl |
应用说明
| 检测时间 | 1.5 h, 30 min |
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属性
| 形式 | 96 well |
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| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | for laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 背景介绍 | Estradiol (1,3,5(10)-estratriene-3,17β-diol; 17β-estradiol; E2) is a C18 steroid hormone with a phenolic A ring. This steroid hormone has a molecular weight of 272.4. It is the most potent natural Estrogen, produced mainly by the Graffian follicle of the female ovary and the placenta, and in smaller amounts by the adrenals, and the male testes. Estradiol (E2) is secreted into the blood stream where 98% of it circulates bound to sex hormone binding globulin (SHBG) and to a lesser extent to other serum proteins such as albumin. Only a small fraction circulates as free hormone or in the conjugated form. Estrogenic activity is affected via estradiol-receptor complexes which trigger the appropriate response at the nuclear level in the target sites. These sites include the follicles, uterus, breast, vagine, urethra, hypothalamus, pituitary and to a lesser extent the liver and skin. |
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| 生物功能 | In non-pregnant women with normal menstrual cycles, estradiol secretion follows a cyclic, biphasic pattern with the highest concentration found immediately prior to ovulation. The rising estradiol concentration is understood to exert a positive feedback influence at the level of the pituitary where it influences the secretion of the gonadotropins, follicle stimulating hormone (FSH), and luteinising hormone (LH), which are essential for folicular maturation and ovulation, respectively. Following ovulation, estradiol levels fall rapidly until the luteal cells become active resulting in a secondary gentle rise and plateau of estradiol in the luteal phase. During pregnancy, maternal serum Estradiol levels increase considerably, to well above the pre-ovulatory peak levels and high levels are sustained throughout pregnancy. Serum Estradiol measurements are a valuable index in evaluating a variety of menstrual dysfunctions such as precocious or delayed puberty in girls and primary and secondary amenorrhea and menopause. Estradiol levels have been reported to be increased in patients with feminising syndromes, gynaecomastia and testicular tumors. In cases of infertility, serum Estradiol measurements are useful for monitoring induction of ovulation following treatment with, for example, clomiphene citrate, LH-releasing hormone (LH-RH), or exogenous gonadotropins. During ovarian hyperstimulation for in vitro fertilisation (IVF), serum estradiol concentrations are usually monitored daily for optimal timing of human chorionic gonadotropin (hCG) administration and oocyte collection. |
| 产品亮点 | Related products: Estradiol antibodies; Estradiol ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 研究领域 | Neuroscience kit |
检测图片 (1) Click the Picture to Zoom In
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ARG80839 Human Estradiol ELISA Kit example of standard curve image
ARG80839 Human Estradiol ELISA Kit results of a typical standard run with optical density reading at 450 nm.
| Title | Download Link |
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| ARG80839 Human Estradiol ELISA Kit User's manual |
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