ARG70304
Human EGF (pro) recombinant protein (Active) (Fc-His-tagged, C-ter)
Human EGF (pro) recombinant protein (Active) (Fc-His-tagged, C-ter) for Binding Activity,Functional study,SDS-PAGE and Human
概述
| 产品描述 | HEK293 expressed, Fc-His-tagged (C-ter) Active Human EGF (pro) recombinant protein. |
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| 反应物种 | Hu |
| 应用 | Binding, FuncSt, SDS-PAGE |
| 靶点名称 | EGF (pro) |
| 物种 | Human |
| A.A. 序列 | Asn971 - Arg1023 of Human pro - EGF (NP_001954.2) with an Fc - 6X His tag at the C - terminus. |
| 表达系统 | HEK293 |
| 生物活性 | Active |
| 活性说明 | Measured by its ability to stimulate EGF Receptor autophosphorylation in A431 cells. 1-10 ng/ml of Recombinant Human EGF can effectively enhance EGF Receptor autophosphorylation. Measured in a cell proliferation assay using BALB/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.065-0.26 ng/ml. |
| 別名 | Urogastrone; Pro-epidermal growth factor; URG; HOMG4; EGF |
应用说明
| 应用说明 | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Human EGFR at 5 ug/ml (100 µl/well) can bind Human EGF with a linear range of 7-25ng/ml. |
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属性
| 形式 | Powder |
|---|---|
| 纯化说明 | 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 90% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 复溶 | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 基因名称 | EGF |
|---|---|
| 全名 | epidermal growth factor |
| 背景介绍 | This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016] |
| 生物功能 | EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). [UniProt] |
| 细胞定位 | Membrane; Single-pass type I membrane protein. [UniProt] |
| 预测分子量 | 134 kDa |
| 翻译后修饰 | O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor). [UniProt] |
检测图片 (1) Click the Picture to Zoom In
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ARG70304 Human EGF (pro) recombinant protein (Active) (Fc-His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70304 Human EGF (pro) recombinant protein (Active) (Fc-His-tagged, C-ter).
