ARG81868

Human DLL1 ELISA Kit

Human DLL1 ELISA 试剂盒 for ELISA and Human

内含物

货号 内含物名称 包装 温度
ARG81868-001 Antibody-coated microplate 8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81868-002 Standard 2 X 10 ng/vial 4°C
ARG81868-003 Standard/Sample diluent 30 ml (Ready to use) 4°C
ARG81868-004 Antibody conjugate concentrate (100X) 1 vial (100 µl) 4°C
ARG81868-005 Antibody diluent buffer 12 ml (Ready to use) 4°C
ARG81868-006 HRP-Streptavidin concentrate (100X) 1 vial (100 µl) 4°C
ARG81868-007 HRP-Streptavidin diluent buffer 12 ml (Ready to use) 4°C
ARG81868-008 25X Wash buffer 20 ml 4°C
ARG81868-009 TMB substrate 10 ml (Ready to use) 4°C (Protect from light)
ARG81868-010 STOP solution 10 ml (Ready to use) 4°C
ARG81868-011 Plate sealer 4 strips Room temperature

概述

产品描述 ARG81868 Human DLL1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human DLL1 in serum, plasma (heparin, EDTA), urine, human milk and cell culture supernatants.
反应物种 Hu
应用 ELISA
特异性 There is no detectable cross-reactivity with other relevant proteins.
靶点名称 DLL1
偶联标记 HRP
偶联标记说明 Substrate: TMB and read at 450 nm.
灵敏度 39 pg/ml
样品类型 Serum, plasma (heparin, EDTA), urine, human milk and cell culture supernatants.
标准范围 78 - 5000 pg/ml
样本量 100 µl
精确 Intra-Assay CV: 4.2%; Inter-Assay CV: 4.9%
別名 DELTA1; H-Delta-1; Drosophila Delta homolog 1; Delta-like protein 1; DL1; Delta1; Delta

应用说明

检测时间 ~ 5 hours

属性

形式 96 well
存放说明 Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项 For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接

GeneID: 28514 Human DLL1

Swiss-port # O00548 Human Delta-like protein 1

基因名称 DLL1
全名 delta-like 1 (Drosophila)
背景介绍 DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. It may play a role in cell-to-cell communication. [provided by RefSeq, Jul 2008]
生物功能 Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. [UniProt]
产品亮点 Related products:
DLL1 antibodies; DLL1 ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
翻译后修饰 Ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation (By similarity). Ubiquitinated; promotes recycling back to the plasma membrane and confers a strong affinity for NOTCH1. Multi-ubiquitination of LYS-613 by MIB1 promotes both cis and trans-interaction with NOTCH1, as well as activation of Notch signaling. Ubiquitinated by NEURL1B (By similarity).

Phosphorylated in a membrane association-dependent manner. Phosphorylation at Ser-697 requires the presence of Ser-694, whereas phosphorylation at Ser-694 occurs independently of the other site. Phosphorylation is required for full ligand activity in vitro and affects surface presentation, ectodomain shedding, and endocytosis.

O-fucosylated. Can be elongated to a disaccharide by MFNG. [UniProt]

检测图片 (1) Click the Picture to Zoom In

  • ARG81868 Human DLL1 ELISA Kit standard curve image

    ARG81868 Human DLL1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.

Title Download Link
ARG81868 Human DLL1 ELISA Kit User's manual download icon Download